MaizeGDB Docs: Maize Gene Discovery Project: RescueMu Library Plates: Progress

Project Documentation & Protocols: Maize Gene Discovery Project: RescueMu Library Plates: Progress

Progress Report of Library Plate Information and Availability

Grid	Available?	Rows	Columns	Where Grown	% Transposition
G	bacterial only	46	48	Stanford	66
H	yes	38	36	Berkeley	62
I	yes	38	34	Berkeley	N/A
J	yes	38	45	UCSD	38
K	yes	30	30	Stanford	66
L	yes	36	20	Berkeley	66
M	no	40	40	Arizona	125
N	no	32	44	Berkeley	20
O	no	47	48	Stanford	50
P	yes	48	48	Hawaii	140
Q	no	48	24	Hawaii	100
R	in progress	48	48	UCSD	N/A
S	yes	48	48	Arizona	N/A
T	in progress	48	46	Hawaii	140
U	yes	48	48	Hawaii	124
AA	yes	48	48	Berkeleyi	100
BB	yes	34	48	Stanford	N/A
V	no	N/A	N/A	Arizona	N/A
X	no	N/A	N/A	UCSD	N/A

Note: Grids A through S utilized a male founder(s) with transposed RescueMu elements to cross onto multiple recipient ears; the founder was crossed by a standard line. Therefore these grids are composed of mainly pollen-transmitted events with about 10% of the grid individuals from female transmitted events. In Grids AA and BB siblings with newly transposed RescueMu elements were intercrossed to generate populations with a better representation of female-transmitted events. The double letter denotes that both parents had transposed RescueMu elements.

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